Analyzing the oral metagenome

Novel tetracycline resistance determinant from the oral metagenome.

A major drawback of most studies on how bacteria become resistant to antibiotics is that they concentrate mainly on bacteria that can be cultivated in the laboratory. In the present study, we cloned part of the oral metagenome and isolated a novel tetracycline resistance gene, tet(37), which inactivates tetracycline.

Probing Oral Microbial Functionality – Expression of spxB in Plaque Samples

The Human Oral Microbiome Database (HOMD) provides an extensive collection of genome sequences from oral bacteria. The sequence information is a static snapshot of the microbial potential of the so far sequenced species. A major challenge is to connect the microbial potential encoded in the metagenome to an actual function in the in vivo oral biofilm. In the present study we took a reductionist...

Analysis of Metagenome Composition by the Method of Random Primers

Metagenome, a mixture of different genomes (as a rule, bacterial), represents a pattern, and the analysis of its composition is, currently, one of the challenging problems of bioinformatics. In the present study, the possibility of evaluating metagenome composition by DNA-marker methods is investigated. These methods are based on using primers, short nucleic acid fragments. Each primer picks ou...

Title Using the Metagenomics RAST server (MG-RAST) for analyzing shotgun metagenomes Source

Functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis

B Health active Figure S1. Statistical differences in metagenome composition. Metagenome hit counts were first normalized using GASIC. Normalized counts were then analyzed using LEfSe with default parameters, to identify significant differences at species level between the microbial communities compared. a) Comparison non-progressing site baselines to healthy sites of healthy patients b) Compar...